Fowler, ranked 12th in the world, joins a field that features 16 of the top 25 players in the world.
Also playing are Rory McIlroy (1), Sergio Garcia (7), Justin Rose (8), U.S. Open champion Martin Kaymer (11), Hideki Matsuyama (14), Patrick Reed (15), Victor Dubuisson (16), Billy Horschel (17), Phil Mickelson (18), Brooks Koepka (19), Graeme McDowell (20), Dustin Johnson (21), Zach Johnson (22), Chris Kirk (23) and Jamie Donaldson (25).
Fowler's high ranking was earned, in large part, by his superb play in last year's majors.
"I really wanted to win," Fowler said in a statement released by the Honda Classic. "The goals going into the year, I took care of everything except for getting the win. I think some of the big ones were obviously making the Ryder Cup team, playing through the FedEx Cup playoffs and being in contention in all four majors. It may be the only time in my career I'm ever able to do that.
"If I would have won, it would have been almost too perfect knocking off all my goals. So it gives me something to build on and hopefully I can have a season in the near future where I check off all the boxes. A win is definitely in order," he added.