The somewhat odd situation arose when the world number one was left with two choices when her second shot there went well over the hole and her ball came to a stand-still in close proximity of the tree.
She was left with two options – either chip under it, or lob over it.
After deciding on the latter, the New Zealander was shocked to find that the ball came to a rest inside the tree.
As a result, her caddie Jason Hamilton climbed up the tree in an attempt to retrieve it, but after a few minutes of shaking the branch in question, the ball never came down and Ko had to settle for a triple bogey.
At the next hole, the 18-year-old had to settle for a double bogey when her approach found the water trap and a round of two-under quickly become one of three-over. One more dropped shot followed and Ko had to settle for a disappointing four-over 75 which leaves her in serious danger of missing the cut just a week after winning at the Swinging Skirts Classic.
"Those two holes I was 5-over-par, so what can you do?" Ko said from the clubhouse after the round.
"I was going to hit it over that tree, I would have never imagined for it to be stuck up there. Yeah, you just get those days where the things that you least expect happens. Can't do much. On the next hole I didn't really hit a good tee shot, and then it's kind of tough when you're hitting out of the rough trying to get over water, and I put myself in the worst position for there. Just those two holes, it was tough.
"I didn't know he [Hamilton] was that good at climbing trees and monkey bars, so he must have done that when he was in elementary school, but that's a while ago, too. It's good to know that my caddie is always there to support me and to do what's best for me. At the end of the day he didn't really need to go up the tree."
In relation to the incident at the 14th hole, Hamilton admitted that he was a tad nervous with the prospect of getting down from the tree again.
"It was a little hairy," Hamilton said.
"Once I was up there, I didn't think about getting down until I was up there. I just had to figure it out on the fly.
"I haven't got up that high before. That was probably a new record for me. I'm too old for climbing trees.
"I was hanging on pretty tight. I didn't want to fall out."