Serena Wiliams will take on Svitolina, who she has met and beat once before, at the Rod Laver Arena. Williams will be the favourite to win the match and book her place in the next round. Serena's older sister Venus will be facing off against Italian qualifier Camila Giorgi for the first time. Venus's form has been dominant so far during the tournament, and if the Italian were to win, it would be a major upset.
One of the most exciting matches on Saturday will be the one between top seed Novak Djokovic and Spaniard Fernando Verdasco. With Roger Federer knocked out on Friday, the Serb is the clear favourite to win in Melbourne, however getting past Verdasco may prove challenging. Djokovic has struggled against Verdasco in the past, and their head-to-head of 6-4 only slightly favours the World number one.
Also in action in the men's draw is Japanese fan favourite Kei Nishikori. Nishikori will be facing off against Steve Johnson, whom he defeated in straight sets a few weeks ago in Brisbane.
Australian Open: Order of play for Saturday 24 January:
(All times local, 11 hours from GMT)
Rod Laver Arena (11:00AM start) – Varvara Lepchenko (USA)  vs. Agnieszka Radwanska (POL) ; Serena Williams (USA) vs. Elina Svitolina (UKR) ; Stan Wawrinka (SUI)  vs. Jarkko Nieminen (FIN)
Rod Laver Arena (19:00 PM start) – Novak Djokovic (SRB)  vs. Fernando Verdasco (ESP) ; Petra Kvitova (CZE) vs. Madison Keys (USA)
Margaret Court Arena (11:00AM start) – Camila Giorgi (ITA) vs. Venus Williams (USA) ; Feliciano Lopez (ESP)  vs. Jerzy Janowicz (POL); Barbora Zahlavova Strycova (CZE)  vs. Victoria Azarenka (BLR)
Margaret Court Arena (19:00 start) – David Ferrer (ESP)  vs. Gilles Simon (FRA) ; Bob Bryan (USA) / Mike Bryan (USA)  vs. Carlos Berlocq (ARG)/ Leonardo Mayer (ARG)
Hisense Arena (11:00AM start) – Garbine Muguruza (ESP)  vs. Timea Bacsinszky (SUI); Benjamin Becker (GER) vs. Milos Raonic (CAN) ; Coco Vandeweghe (USA) vs. Madison Brengle (USA); Steve Johnson (USA) vs. Kei Nishikori (JPN) 
Show Court 2 (11:00AM start) – Todd Woodbridge (AUS)/ Mark Woodforde (AUS) vs. Henri Leconte (FRA); Mark Philippoussis (AUS); Dominika Cibulkova (SVK)  vs. Alize Cornet (FRA) ; Vasek Pospisil (CAN)/ vs. Guillermo Garcia-Lopez (ESP); Mary Joe Fernandez (USA)/ Iva Majoli (CRO) vs. Nicole Bradtke (AUS)/ Barbara Schett (AUT); Mansour Bahrami (IRI)/ Fabrice Santoro (FRA) vs. Goran Ivanisevic (CRO)/ Ivan Ljubicic (CRO)
Show Court 3 (11:00AM start) – Sara Errani (ITA) / Roberta Vinci (ITA)  vs. Mona Barthel (GER)/ Mandy Minella (LUX); Jeremy Chardy (FRA)/ Lukasz Kubot (POL) vs. Alex Bolt (AUS)/ Andrew Whittington (AUS); Eric Butorac (USA) /Sam Groth (AUS)  vs. Pablo Andujar (ESP)/ Dustin Brown (GER); John Isner (USA)  vs. Gilles Muller (LUX)