Long jumper Darya Klishina has been banned from competing in the Rio Games.
Russian Klishina was the only athlete to survive a blanket ban for doping imposed over the country earlier this year.
Her suspension relates to new evidence revealed by an investigation into allegations that Russia orchestrated a state-sanctioned cheating program.
Based in the United States rather than her nation of birth, the 25-year-old had been afforded an exemption to compete in Brazil, but the International Association of Athletics Federations has since revoked the allowance.
"We have withdrawn her exceptional eligibility status which enables her to compete in international competitions based on new information that has been received," an IAAF official told Reuters.
Klishina responded via social media: "I am a clean athlete and have proved that already many times and beyond any doubt. Based in the U.S. for three years now, I have been almost exclusively tested outside of the anti-doping system in question.
"I am falling victim to those who created a system of manipulating our beautiful sport and is guilty of using it for political purposes. I cannot help but feel betrayed by a system that is not focused on keeping the sport clean and supporting rank-and-file athletes, but rather seeking victories outside sport arenas."
While reasons behind Klishina's ban remain unclear, scratches and scuff marks were reportedly found on her sample containers.