Like most other Honda riders at Le Mans last time out, reigning MotoGP champion Marquez struggled with his machine's front end during the race and dropped from pole position to finish fourth.
"We are very motivated after the weekend in Le Mans and arrive in Italy in good shape," Marquez told crash.net.
"We must work hard from Friday to find a good set-up as Mugello is a complicated circuit, however, I like it and have good memories from my first victory in 2010."
Marquez and Le Mans winner Jorge Lorenzo were involved in a tight battle at Mugello last year, which the reigning champion eventually won in what was the sixth of 10 consecutive victories for the Spaniard.
The 2015 season has been much different for the two-time champion, though. So far, he has only one victory to his name and trails Championship leader Valentino Rossi by 33 points.
"We know Valentino will be very strong at his home race, Jorge and Dani [Pedrosa, his Honda team-mate] have a good history there and Ducati were testing there just a few weeks ago, so we will have to be focused all weekend," Marquez added.