The sides that kick-off their ACL campaigns next week were all in action on Thursday.
Tractorsazi Tabriz enjoyed a 2-1 away win at Naft Masjed Soleyman, Persepolis played out a goalless draw with Saba Qom while Foolad Khouzestan went down to their second straight defeat, with the Iranian champions slipping to a 3-1 loss at Saipa.
Tractorszai geared up for their Champions League Group D opener against Nasaf on Wednesday by coming from a goal down to defeat last season's Azadegan League champions Naft Masjed Soleyman.
Hamid Bou Hamdan opened the scoring with an 11th minute penalty before Saman Nariman Jaman got his second goal in as many games with 12 minutes of the first half remaining.
Brazilian striker Edinho took his tally for the season to 11 when the forward fired home the 83rd winner.
Tractorsazi are now on the league table, one point behind leaders Naft Tehran and one more than third-placed Esteghlal who have a game in hand over the top two.
Reigning champions Foolad remain on 37 points after going down 3-1 away to mid-table Saipa on Thursday.
Foolad's second straight domestic defeat following last week's 1-0 loss to 2007 ACL runners-up Sepahan.
Foolad will be looking to find the winning formula when they host 2011 continental club champions Al Sadd in their opening match in Group C of this year's ACL on Wednesday.
Persepolis had to settle for a share of the spoils after being held to a goalless draw at home by Saba Qom.
It was their last match before they mark their return to Asian club football's premier competition for the first time since 2012 when they host Qatar champions Lekhwiya on the same day Foolad face Al Sadd.