The 116th-ranked Ukrainian caused one of the biggest shocks in the history of the tournament when he beat the seven-time champion on Wednesday, ending the Swiss player's hopes of making it 37 grand slam quarter-finals in a row in the process.
Stakhovsky has previously played with few eyes on him at the All England Club, but he is sure to be well watched when he meets Jurgen Melzer today.
And, with a new legion of admirers, the 27-year-old wants to put on a good display.
"I hope I can come out with a great performance and show what I can do again," he said.
"That's what you have to do in order to win. Winning against Federer was great, but I only won in the second round. There are another five rounds."
Should he win, Stakhovsky will reach the fourth round of a grand slam for the first time in his career.
Elsewhere in his half of the draw, Andy Murray will look to ignore talk suggesting he essentially has a bye through to the final when he meets the dangerous Tommy Robredo on Centre Court, while the well-regarded duo of Nicolas Almagro and Jerzy Janowicz square up.
There is also the chance for the German-Jamaican Dustin Brown - a man who has made quite the impression on the Wimbledon fans owing to his dreadlocked appearance and colourful story - to make the fourth round when he meets Adrian Mannarino.
Other matches include some unfinished business from yesterday as Bulgarian Grigor Dimitrov returns to court 9-8 down and serving in a deciding set against Grega Zemlja.
They were thwarted by the weather which struck at the most unfortunate of times, while David Ferrer did not even start his match with Roberto Bautista Agut, but a Centre Court billing ensured Novak Djokovic was given the chance to progress under the dry of the roof.
He made sure he took it, beating Bobby Reynolds in three sets, with the result meaning that no American man has made it through to the third round of the tournament for the first time since 1912.
Elsewhere there were impressive wins for Juan Martin del Potro against Jesse Levine and Tomas Berdych over Daniel Brands.
Milos Raonic, the big-serving Canadian who was once tipped for big things, suffered a straight-sets loss to Igor Sijsling, while Richard Gasquet looked very good in seeing off Go Soeda.
There was a bizarre story, as Michael Llodra walked out of his match with Andreas Seppi citing a hamstring injury, only to play doubles with fellow Frenchman Nicolas Mahut less than four hours later, with the duo progressing when opponent Jaroslav Levinsky hurt his back and had to withdraw.