The 23-year-old eighth seed landed her only grand slam singles title to date at the All England Club in 2011, overcoming Maria Sharapova in the final, and her performances since then have been inconsistent.
Russian third seed Sharapova saw her chances come to an abrupt end on Wednesday, the same day second seed Victoria Azarenka was forced to pull out through injury.
As a result, some bookmakers have made Kvitova second favourite for the title and she continues her campaign with what looks a difficult assignment against Russian 25th seed Ekaterina Makarova.
Kvitova is the only player top 10 seed left in the bottom half of the draw, although the top half is more competitive.
Top-seeded Serena Williams is the pick of the bunch and on a 33-match winning streak after yesterday beating France's Caroline Garcia 6-3 6-2 in round two.
Williams will next face Japan's Kimiko Date-Krumm, who at the age of 42 became the oldest woman to reach the Wimbledon third round in the open era by beating Romania's Alexandra Cadantu.
Date-Krumm had a 12-year break from the game that ended in 2008 and is arguably playing close to the form that took her through to the 1996 Wimbledon semi-finals.
"She is incredibly inspiring and she is so fit," Williams said. "She has done really well.
"I've never played her before. I watched her when I was super young growing up. I know Venus has played her so I will definitely be talking to her over the next couple of days.
"It is unbelievable. I don't know how she is able to do so. She obviously stopped for a little while but coming back and being so great is really inspiring."
Williams beat Agnieszka Radwanska in last year's final and the Polish fourth seed yesterday overcame Mathilde Johansson 6-1 6-3, setting up a third-round tussle with American 18-year-old Madison Keys.
Petra Martic, Tsvetana Pironkova and Klara Zakopalova also collected wins, as did sixth seed Li Na and 11th seed Roberta Vinci.
Sabine Lisicki set up a clash with 2011 US Open winner Samantha Stosur, while Dominika Cibulkova and Marina Erakovic also won through.
Germany's Angelique Kerber and Britain's Laura Robson were among those who saw matches cancelled due to rain and rescheduled for.