Second seed Victoria Azarenka, men's title fancy Jo-Wilfried Tsonga and Rafael Nadal's conqueror Steve Darcis among the names to head through the exit door due to a variety of problems.
Marin Cilic, John Isner, Radek Stepanek and Yaroslava Shvedova were others to depart, while the likes of Caroline Wozniacki and Maria Sharapova also suffered on the grass suface in SW19 as they slipped around out on Court Two.
Seven is believed to be the largest number of withdrawals at a Grand Slam on a single day in the history of Open era tennis.
It was an injury list which saw Azarenka call on the All England Club to investigate.
She criticised the state of the courts after she was forced to withdraw following a heavy fall.
Azarenka suffered a knee injury during her first-round match on Monday and withdrew minutes before she was due on court for Wednesday's clash with Flavia Pennetta.
Asked if there was anything different with the courts this year, the 23-year-old said: "I'm wondering the same question, because the court was not in a very good condition on Monday.
"My opponent fell twice; I fell badly; there were some other people who fell after.
"So I don't know if it's the court or the weather. I can't figure it out it.
"It would be great if the club or somebody who takes care of the court just would examine or try to find an issue so that wouldn't happen.
"Because on something like this, there is nothing I could have done to make that better. There is nothing I've done wrong that cost me to just withdraw from Wimbledon."
However, tournament officials were quick to state: "There has been no change to the way we prepare the courts.''
Wozniacki suffered an ankle injury during her defeat to Petra Cetkovska, while Sharapova was also falling all over the place during her match with Michelle Larcher de Brito.
Tsonga, considered a dark horse for the title by many, suffered knee and wrist problems after falling during his clash with Ernests Gulbis and quit when two sets to one down.
The Frenchman would not blame the Centre Court grass for his departure though, citing a tendon injury picked up last week.
"There is nothing about this court. They're great,'' he said.
Tsonga joined Darcis in heading home - he withdrew having picked up a shoulder injury during his stunning first-round victory over Nadal.
The Belgian recalled a diving shot in the seventh game of the opening set against Nadal, believing it was where his injury stemmed from.
"After the match, a few hours after, I started to feel so much pain," he said. "I couldn't sleep at night. I saw the physio and the doctor and they did a good job. It's a little better today but there is no chance I can play.
"I cannot serve, even on the forehand serve. I cannot hit a ball."
Meanwhile, Isner jarred his knee early in his clash with Adrian Mannarino and quit after two games, while Stepanek withdrew from his match with 24th seed Jerzy Janowicz due to a hamstring problem while 6-2 5-3 down.
Like Darcis, Cilic and Shvedova didn't even make it out of the locker room. Cilic, the 12th seed and recent Queen's Club finalist, cited a left knee injury for his decision to pull out of his scheduled clash with Kenny De Schepper, while Shvedova's withdrawal due to a right arm injury saw former champion Petra Kvitova progress without hitting a ball.