World number three Garbine Muguruza crashed out of the US Open as she was stunned in round two by Latvia's Anastasija Sevastova.
Former world number one Caroline Wozniacki came from 4-0 down in the first set to dump ninth seed Svetlana Kuznetsova out of the US Open.
Milos Raonic's hopes of a maiden grand slam title were brought to a grinding halt by Ryan Harrison on Wednesday.
French ninth seed Jo-Wilfried Tsonga needed four sets to get past James Duckworth of Australia to reach the third round at the US Open on Wednesday.
Top seed Novak Djokovic reached the third round of the US Open when Jiri Vesely of the Czech Republic was forced to withdrawal due to a left forearm injury on Wednesday.
Seventh seed Roberta Vinci, Petra Kvitova and Belinda Bencic all won their second round matches at the US Open in Flushing Meadows, New York on Wednesday.
Ahead of a potential record-breaking grand slam, Nike put together a heartwarming video for Serena Williams.
Despite another early exit at a grand slam, Ana Ivanovic is not considering hanging up her racket just yet.
Ivo Karlovic broke the record for the most aces served in a US Open match during his win over Yen-Hsun Lu on Tuesday.
Eugenie Bouchard crashed out in the US Open first round but insisted she has not been distracted by her ongoing lawsuit against the event.
Andy Murray was not troubled in his US Open opener on Tuesday as he beat Lukas Rosol 6-3, 6-2, 6-2 to progress to the second round.
It was business as usual for world number one Serena Williams in the first round of the US Open on Tuesday as she faced little trouble in her 6-3, 6-3 victory over Ekaterina Makarova.
Australian bad boy Bernard Tomic has caused a stir at the US Open after he was caught directing a lewd comment at a spectator.
Stan Wawrinka and David Ferrer eased into the second round of the US Open on Tuesday after routine wins.
Kei Nishikori and Grigor Dimitrov advanced to the second round of the US Open following their respective wins on Tuesday.
Simona Halep eased past Kirsten Flipkens while Ana Ivanovic was knocked out in the opening round of the US Open on Tuesday.
Spanish third seed Garbine Muguruza has played down her US Open chances after her opening round win.
Novak Djokovic celebrated getting through his opening round clash at the US Open on Monday by doing a song and dance routine.
World number one Novak Djokovic was made to fight in his first round match at the US Open on Monday, eventually downing Jerzy Janowicz 6-3, 5-7, 6-2, 6-1.
Andy Murray winning the US Open could prove a turning point in his rivalry with Novak Djokovic, says former British number one Greg Rusedski
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