The former Olympic champion was last seen being knocked unconscious by British and Commonwealth champion David Price in October.
But, now 41, Harrison still hopes to leave the sport on a high despite numerous disappointing performances.
One of those came against Northern Ireland's Martin Rogan, who - like Harrison - is a former Prizefighter champion who will be looking to add a second trophy to his collection.
Rogan, also 41, went five rounds with Tyson Fury last April but it is almost five years since he burst on the domestic heavyweight scene with victory in the very first Prizefighter at this venue.
The veteran will make his comeback in a four-round warm-up fight in Belfast on February 9.
Ironically, Prizefighter provided Harrison with a springboard to a world title fight on the back of a narrow loss to Rogan in 2008.
Within a year, he had won the European title against Michael Sprott with a desperate late knockout before a lamentable display against then-WBA champion David Haye.
Brixton's Ian Lewison and American Travis Walker have also been confirmed as participants in the unique eight-man, one-night tournament, while Essex-based Pole Albert Sosnowski is expected to add his name to the list.
In addition, former Team GB captain Tom Stalker will make his professional debut on the undercard.
The tournament kicks-off a massive night of boxing on Sky Sports with Kell Brook challenging IBF welterweight champion Devon Alexander in Detroit later in the evening.
"The heavyweights edition of Betfair Prizefighter is always the most popular and with these colourful characters and former champions we are expecting an instant sell out at York Hall," said promoter Eddie Hearn.
"I'm also delighted that the GB Olympic captain will make his professional debut on the show and will be live on Sky Sports."