Chambers, who is now eligible for selection for the London 2012 Olympics after the British Olympic Association's lifetime ban for drugs cheats was ruled non-compliant, had been invited to the May 25 event prior to that verdict being reached by the Court of Arbitration for Sport (CAS) earlier this week.
Alfons Juck, the meeting manager for Golden Spike, told Press Association Sport: "I can confirm that Dwain Chambers has been invited to the Golden Spike event.
"At the start of the year his agent and lawyer approached all the meetings, including Ostrava. We just waited for the announcement, I wanted to see how it would end with the CAS situation, with the BOA and so on and then we published it.
"But it had nothing to do with the decision over whether Dwain would run in Ostrava or not. We just waited because we thought from a PR point of view it would be more wise to wait, then put it into the right perspective."
Chambers served a two-year ban after testing positive for a banned steroid in 2003.
In addition to Chambers and Bolt, the meeting currently will feature three other men who have gone under 10 seconds - American Darvis Patton, Jamaican Lerone Clarke and Zimbabwe's Ngonidzashe Makusha.
Britain's Harry Aikines-Aryeetey has also been invited to compete in the race.