Repsol Honda rider Pedrosa, 27, has been in the form of his life so far this season, and holds a seven-point advantage over Yamaha's reigning world champion Jorge Lorenzo.
But Pedrosa has had to watch Lorenzo eat into his lead with back-to-back victories in Italy and Catalunya, and will not want his fellow Spaniard to get any closer in when the historic Assen track hosts a round of the world championship for the 65th time.
Pedrosa, a solid second last time out in Barcelona, has never won a top class race at a track dubbed 'the cathedral of speed', but is inspired by memories of his triumph in the 125cc class way back in 2002.
"After a good race in Catalunya and an important 20 points, I still lead the championship and I am looking forward to Assen," he said.
"I won my first ever race there and have very special memories.
"The weather can be temperamental so I hope we get some dry track time on Friday to find a good setting. It is a good track but it's important to have a good feeling with the tyres because there are several fast corners.
"I hope we can keep the momentum going and do a good race"
Rival Lorenzo, meanwhile, is confident of keeping his winning run going at a track which is his favourite on the calendar.
"We were in a very good shape these last two races and I feel stronger than ever and really hungry to win," he said.
"I think in the last two races we made a quite big step on the bike to get the consistency on race pace.
"Assen has been my favourite track ever. I love the circuit, it is historic, quick and very flowing, but also I love the landscape, the ambience and I have always liked the country.
"I am very motivated to go there and try to keep this run of wins going."