The 32-year-old Spaniard was taking part in her first test for the Formula One team at Duxford Airfield when she crashed into a support truck after completing her opening installation lap.
After treatment by trackside paramedics, De Villota was taken to Addenbrooke's Hospital in Cambridge where it is understood she has been receiving treatment for head and face injuries.
The latest Marussia team statement read: "The team can confirm Maria was transferred by ambulance from Duxford Airfield, where the accident happened, to Addenbrooke's Hospital in Cambridge.
"Since Maria's arrival at the hospital at approximately 10.45hrs this morning, she has been receiving the best medical attention possible at the hospital, which is the region's major trauma centre.
"Maria is conscious and medical assessments are ongoing. The team will await the outcome of these assessments before providing further comment.
"The team's first priority at this time is Maria and her family."
De Villota, daughter of retired Formula One driver Emilio, was conducting a straight-line aero test at the old Second World War site.
Early theories behind the accident have suggested the anti-stall mechanism on the car kicked in as she approached a makeshift pit area, propelling her into the truck at around 20-30mph.
It is understood the tailgate had been lowered at the time, and at such a height as for De Villota to sustain head trauma.
With emergency services at the scene, De Villota was attended to quickly, initially by fire crews and soon after paramedics.
Although it is believed she was unconscious for around 15 to 20 minutes, Press Association Sport understands De Villota at least regained consciousness and was talking prior to being transferred to Addenbrooke's.
A statement from East of England Ambulance Service, who attended the scene, confirmed receiving an emergency call at 9.17am prior to despatching a crew to Duxford.
Spokesman Gary Sanderson later stated the service had treated a woman who had "sustained life-threatening injuries".
Air ambulance charity Magpas were also on hand to assist, with Dr Darren Reid and volunteer paramedic Dave Mee in attendance.
A statement read: "The Magpas team were called to Duxford Airfield and arrived at 10:20 this morning [Tuesday] after a Formula One car collided (at slow speed) with a lorry tail lift.
"The Helimedix assessed the patient, who suffered facial and head injuries, and administered pain relief as they worked alongside an EEAST ambulance crew who were also at the scene."
In light of the accident, a number of F1 drivers have expressed their concerns and offered messages of support to De Villota via Twitter.
McLaren star Jenson Button said: "Terrible accident for Maria De Villota, Marussia F1 team test driver. My thoughts are with Maria and her family at this very difficult time."
De Villota's compatriot and Ferrari driver Fernando Alonso added: "I just got home and found out Maria's accident, we called the family and hopefully we will know more soon! All my energy with you!"
Toro Rosso driver Daniel Ricciardo tweeted: "Let's put Maria De Villota in our hearts today [Tuesday]."