Ballesteros, Europe's talisman in the event for so long and then captain in his native Spain in 1997, died in May last year after a long battle with a brain tumour.
His partner during most of his cup career was Jose Maria Olazabal, who is the European captain this year, and as well as having the image of Ballesteros on their bags the players will also have his silhouette on their shirts.
"We have Seve's blue and white, Seve on the bag and his right-hand man as our captain hopefully seeing us home," said Ian Poulter, winner of all his three games despite the Americans building a 10-6 lead.
As holders Europe need a 14-14 tie to retain the trophy. That means winning eight and losing only four of the remaining games.