The 22-year-old rookie from Cleethorpes, playing her fourth Tour event, finished with a two-under round of 71 to hold off the challenge of hard-charging compatriot Charley Hull, the 17-year-old who went round today in 69, four under par.
Clyburn, who moved into contention with yesterday's 69, held her nerve after an early bogey, while overnight leader and fellow rookie Camilla Lennarth hit the self-destruct button.
The Swede, who was clinging to a one-shot advantage overnight after following an opening 66 with a round of 73 yesterday, could not rediscover that early form and instead carded three bogeys and a double-bogey in a disappointing 77.
In contrast, Clyburn steadied herself after bogeying the second hole, and back-to-back birdies on the fifth and sixth set her on course for victory.
As Lennarth tumbled down to fourth, Hull made her move, with fourth birdies on the opening nine putting her in contention before two late bogeys ended her chances of catching Clyburn.
Carin Koch also capitalised on her fellow Swede's struggles to take third place with a round of 71, leaving her four shots off the lead, while Lennarth was left to share fourth with Bree Arthur and Carlota Ciganda.