Watson caused a stir at last year's Ryder Cup by encouraging the spectators around the first tee at Medinah to break with golf etiquette by making as much noise as they wanted while he teed off.
Poulter gladly accepted the challenge and did the same, but it promises to be a more traditional scene when he, Watson and US amateur champion Steven Fox tee off at 10:34am local time on Thursday at Augusta National.
World number one Tiger Woods, seeking his fifth Green Jacket, is in the group behind alongside England's Luke Donald and American Scott Piercy, but Rory McIlroy will have to wait until the penultimate match of the day before getting his challenge under way.
McIlroy is out at 1:41pm alongside the man he succeeded as US PGA champion last year, American Keegan Bradley, with Sweden's Fredrik Jacobson completing the group.
American trio Jason Dufner, Matt Kuchar and Bill Haas are last out 11 minutes later, while 1988 champion Sandy Lyle is in the first group out in the tournament proper at 8am with John Peterson and amateur Nathan Smith.
Gary Player, Jack Nicklaus and Arnold Palmer will again act as honorary starters a few minutes earlier, hitting tee shots on the par-four first before retiring to the clubhouse.
Lee Westwood, third last year and second in 2010, has 2003 champion Mike Weir and former US Open champion Jim Furyk for company at 9:06am, while Welsh debutant Jamie Donaldson is out at 11:29am alongside 1998 winner Mark O'Meara and Scotland's Martin Laird, who only secured his place by winning the Texas Open on Sunday.
Chinese schoolboy Guan Tianlang, at 14 the youngest player in Masters history, has been drawn alongside two-time Masters champion Ben Crenshaw - with whom he shared a practice round on Monday - and Italian Matteo Manassero, whose place in the record books he has taken.
Manassero was 16 when he finished 36th here in 2010 and has since gone on to win three European Tour events before his 20th birthday, which is on April 19.
Guan, who qualified by winning the Asian Amateur Championship last year, also played nine holes with Woods on Monday, who was taken aback by the Chinese prodigy.
"It's hard to believe," Woods said.
"When I was 14, I was trying to play more tournaments and I was running track and cross-country, trying to get homework done. I couldn't imagine not just playing in a Tour event, but the Masters.
"I think I was probably longer at the time, but he's so consistent. He was hitting a lot of hybrids into the holes yesterday [Monday], hitting them spot on, right on the numbers. He knew what he was doing, he knew the spots he had to land the ball, and to be able to pull it off.
"From a 14-year-old to be able to come out here and handle himself the way he's done is just unbelievable."