The South African Olympian and Paralympian, who runs on carbon fibre blades, plays off an 18 handicap and will feature in the team competition which sees amateurs competing alongside professionals.
Pistorius, who won two gold medals and a silver at the London Paralympics, said: "I am a big golf fan and love the game.
"I have never played St Andrews, Carnoustie or Kingsbarns before, so playing them in a world-class event is a unique opportunity. There is so much history at St Andrews, every golfer longs to play there."
Other amateurs featuring along with 10 Major winners include actor Greg Kinnear, musician Huey Lewis and former Dutch footballers Ruud Gullit and Johan Cruyff.
Ernie Els and Paul Lawrie are among the professionals who will tee off on Thursday, along with holder Michael Hoey.