It is also the same ground which a year ago witnessed one of their biggest disappointments as Walter Mazzarri recalled: "It was there where we lost the chance to qualify for the Champions League."
A win at the Stadio Dall'Ara would guarantee a place in next season's Champions League, regardless of how third-placed AC Milan fare at Pescara.
Securing a place in Europe's elite club competition could also be enough to persuade striker Edinson Cavani to commit his future to the club.
Having broken through the 100-goal barrier with his eighth hat-trick for Napoli in the weekend win over Inter Milan, it is hardly surprising most of Europe's top clubs are casting an eye over the Uruguayan.
Napoli may struggle to turn down bids for him this summer, but with guaranteed income from Champions League football, it may take a greater sum to make them part with their star player.
"We are within touching distance of our aim and the coach tells us to take things one game at a time," said Cavani.
"We are thinking about the Bologna game. We have three games to go and I will give my best for myself and for this Azzurra shirt.
"I hope I can further improve my record. We deserve the Champions League."
Napoli meet a Bologna side who were stung by a 6-0 defeat to Lazio on Sunday.
"We are all very disappointed, but I am not going to forget all of the good things we have done this season," said their coach Stefano Pioli.
"This was the first time we totally forgot to play and it's an incident that we could have done without."
Nevertheless, with 40 points already, the Rossoblu can go into Wednesday night's game with enough confidence that they will be playing in Serie A again next season.