"I am a great admirer of Francesco and I have been extremely pleased with how hard he has been working," DiBenedetto said at the Leaders in Football conference.
DiBenedetto's comments come on the back of rumours that the relationship between Totti and new manager Luis Enrique had become strained.
"The growth of the team has been especially pleasing. The players have taken to Enrique's style of hard work and team play," DiBenedetto said.
"Everybody from Francesco Totti down has worked very hard. Francesco has probably worked harder than anybody. Unfortunately, he is out [injured] right now.
"Anybody who watched the last couple of matches saw how hard he was playing for nearly 90 minutes and that was very impressive. That set a very good example for a lot of the younger players, who have also been working very hard."
Totti is a doubt for the city derby at Lazio on October 16 after suffering a thigh injury, while DiBenedetto has been excited by the rivalry between the two clubs.
"Obviously, Rome-Lazio is special," he said.
"The other day [sporting director] Walter Sabatani asked me did I understand what Roma-Lazio was. He said, ‘What can you compare it to?' I said, ‘a civil war'.
"I think it is a match between two teams this year who are both very good and extremely competitive. We hope to win, I know both teams will play very hard and it will be very exciting."
He added: "Rome is a city which has universal acceptance and recognition. Most people in the world study about Rome, so it is a name they are familiar with. The historical preservation of the city means it is a place that people come and visit. Virtually everybody in the world one day wants to visit Rome."
"We believe that gives us an advantage to be able to reach out to them. Also, Rome, uniquely, has the most embassies of any city in the world, with the Vatican, Italy and the United Nations. Those people - the diplomats who come to Rome - we would like to make them Roma fans so that when they go back to their countries they will become our diplomats."
And while DiBenedetto admits he's currently pleased with the squad at Enrique's disposal, he was not about to rule out more arrivals in the January transfer window should they be necessary.
"It depends what we need. Frankly, we are quite pleased with the play of a lot of the players and how they have developed.
"We have 14 new players on our team and they have to learn about each other's tendencies and abilities.
"We will see. I think at the moment we are quite happy with the players we have got."
Roma were one of the busiest clubs over the summer, bringing in the likes of Bojan Krkic, Maarten Stekelenburg, Erik Lamela, Miralem Pjanic, Simon Kjaer, Gabriel Heinze and Fernando Gago in a bid to improve on last season's 6th-placed finish.
DiBenedetto was named as the club's president late last month. The 62-year-old American is the first foreign president in Roma's history. He took over from Giorgio Cappelli, who was named as caretaker president in July after the end of the Sensi family's 18-year association with the club.