The islanders have been in the Spanish top flight since 1997 but that long run could well end this weekend.
Mallorca head into the final day bottom of the standings, a point behind Celta Vigo and Zaragoza and two behind 17th-placed Deportivo la Coruna.
Only one of that quartet will be able to secure survival and Mallorca forward Victor Casadesus believes the way to deal with the situation is focus on themselves.
"We have to go to win," he said. "We know that there is only this opportunity and we have to win and we know we have to wait for other parties and other results.
"We have to win and you have to concentrate from the first minute.
"Knowing that there are options, albeit few, we have to be happy."
Victor feels for the Mallorca faithful after what has been a tough season but has called for those at the Iberostar Stadium to stick with them on Saturday.
"I understand you are angry and disappointed," he said in a message to the fans.
"But in the game come to cheer because you never know what can happen. What we have to do is win our game. This nobody has any doubt about."
Valladolid sit 14th heading into the game and, while survival is already secure, they still have something to play for.
It was announced yesterday that coach Miroslav Djukic would leave the La Liga club after two seasons at the helm and the Serbian will want to go out with a bang.
"It is the moment to seek new challenges," he said.
"It was a three-year project but because the players have done things so well, we have completed our objective in two.
"We have consolidated the team in the top flight, playing with our own particular style.
"I am not sure where I am going to go, I am just saying I will not continue."