Barca's hopes of winning a fourth European Cup crown in eight years were left in tatters as Bayern followed up their 4-0 semi-final first leg victory with a similarly impressive triumph at the Nou Camp on Wednesday night to complete a 7-0 aggregate win.
Tito Vilanova's men will now be hoping to banish that painful memory as soon as they can and the Primera Division offers them the perfect chance to do just that.
They go into this weekend's fixtures lying 8 points clear of second-placed Real Madrid with five games remaining, and a victory at home to Real Betis will mean they can seal the title in the next weekend.
Barca midfielder Andres Iniesta said: "The league is very close. The other day I said a season couldn't be a bad one if you win the league. Hopefully we can win it as soon as possible to once again have the feeling of happiness."
On their Champions League exit, the Spain international added: "The reality hurts. It hurts us not to have been at the level we needed to be."
Barca could have claimed the La Liga title last weekend if results had gone their way, but they were held 2-2 at Athletic Bilbao while Madrid subsequently trimmed the gap between the pair by two points with a 2-1 derby win at Atletico Madrid.
Madrid may be resigned to losing their league crown while their own Champions League bid ended in disappointment this week with defeat at the hands of Borussia Dortmund, but that does not mean they will be coasting through to the end of the season.
They still have the Copa del Rey final against Atletico to look forward while in La Liga they want to make Barca earn the title.
Sociedad, who go to Getafe on Monday, are in turn five points clear of Malaga and Valencia in fifth and sixth as the Basque club seek a top-four finish in La Liga for the first time since 2003.
Real Mallorca currently prop up the standings on 28 points but they host a Levante side this weekend who have suffered four successive defeats.
The other two teams in the bottom three, two points above Mallorca, are Real Zaragoza and Celta Vigo.
Zaragoza play host to eighth-placed Rayo Vallecano on Sunday, while Sevilla play mid-table rivals Espanyol.
On Friday, Iago Aspas netted a late equaliser to earn relegation-fighting Celta Vigo a deserved 1-1 draw from an entertaining clash with Athletic Bilbao.
The hosts bounced back from their Champions League semi-final elimination at the hands of Borussia Dortmund to close the gap on leaders Barcelona to eight points ahead of their home match with Real Betis on Sunday.
There are four rounds of matches left after this weekend.
The build-up to the clash with Valladolid was dominated by the future of coach Jose Mourinho and his expected departure to Chelsea, but once the match started it was Ronaldo who again attracted much of the attention.
He helped the hosts recover from conceding an early goal to Oscar, making it 2-1 after Angel Di Maria had levelled.
Javi Guerra equalised before the break for the visitors, but second-half efforts from Kaka and Ronaldo - who also struck the crossbar just before half-time with a stunning 30-yard free-kick - looked to have got Madrid home and dry.
Sastre's brilliant volley got Valladolid a late third, but there was no dramatic equaliser as Madrid ensured arch rivals Barca will have to wait a while longer to take their league crown.
In Saturday's other matches, third-placed Atletico Madrid were held to a goalless draw at lowly Deportivo La Coruna but got the point they needed to be assured of Champions League football next season, while Valencia boosted their hopes of a top-four finish with a 4-0 win over nine-man Osasuna.
Two goals late in the first half from Roberto Soldado and Ricardo Costa gave the hosts a comfortable lead and the second half became a formality after Alejandro Arribas was sent off and Ever Banega scored from the resulting free-kick.
David Timor then also saw red and Jonas added to the visitors' pain by scoring a penalty in injury time.
Valencia's win saw them move to within two points of fourth-placed Real Sociedad, who play Getafe on Monday, and lifted them three points above sixth-placed Malaga as Manuel Pellegrini's men slumped to a 1-0 defeat at Granada, Jude Ighalo scoring the only goal after 28 minutes.
Friday's La Liga Result
Saturday's La Liga Results
Sunday's La Liga Fixtures
Mallorca vs Levante
Getafe vs Real Sociedad
Monday's La Liga Fixture
Celta Vigo vs Atletico Madrid