The 2-1 loss to their arch-rivals at the Camp Nou leaves Guardiola's team seven points adrift off the top of the table and effectively ended their hopes of retaining the La Liga title.
But the former Barcelona midfielder was happy with his side's performance in both the Clasico and the whole season. Barcelona dominated the match registering upto 71% of possession in the second half and they had far more goalscoring opportunities.
He was also happy with the dedication and the commitment showed by his players who were only let down by their poor set piece defending and an inability to properly track Real star Cristiano Ronaldo.
They conceded their first goal after Pepe managed to jump highest and head the ball towards goal from a corner. The shot was saved by Victor Valdes but Sami Khedira managed to bully Carles Puyol off the ball and nod it into the net. In the second half, Ronaldo ensured that his side maintained their advantage as he scored the winning goal.
"It's difficult to do an analysis of the match after so many emotions, but I have the impression that we played a good game against a powerful team," Guardiola stated.
"We controlled their counterattacks well and they didn't create a lot of danger.
"There are very few things I can blame on the team. We've been on this path for a long time.
"We had a lot of pressure on us starting in August and we haven't stopped. We can't expect to always be up to the standard, but it's a pity that we faltered in the decisive moment.
"We gave it our all."
Vice-President Josep Maria Bartomeu echoed the coach's words and believes that the season was still successful despite the possibility of Barcelona failing to reclaim their title.
"The team dominated [Real] and we created goal scoring chances. This season has been magnificent nonetheless," he said.
The Blaugrana now have to turn their attention to Europe as they play Chelsea on Tuesday looking to overturn their one-goal deficit from the Champions League semi-final first leg.
"I'll talk to my players on Sunday when things have calmed down, we'll focus on next week - which means we have to focus on Chelsea," Guardiola said.
"We hope to recover well and play the game against Chelsea."
"We will see on Tuesday whether we will pass this test, but I think (the players) will lift themselves up and look at the manner of how things will go against Chelsea.
"What happens against Chelsea will mark the rest of our season. Although the guys have not had long to recover, they have had setbacks like this before and risen to the challenge."
Barcelona could have an opportunity to exact revenge if the two Spanish teams manage to qualify for the Champions League final. But that would require Real to beat Bayern Munich at the Santiago Bernabeu on Wednesday as Los Merengues lost their first leg earlier in the week as well.