Reports in Spain suggested big-spending City had tabled 50million euros plus striker Mario Balotelli to bring Argentina ace Messi to the Etihad Stadium but Barca had rejected the bid.
However, Bartomeu insists no such offer was made.
He told Sport: "Our policy is not to sell our stars but on this subject it certainly did not (happen)."
Meanwhile, Bartomeu made no guarantees over the future of Barca coach Pep Guardiola but hopes the Spaniard will stay on at Camp Nou.
The Barca chief added: "The decision lies with Pep but we hope to go on with him on the bench."