A dramatic 4-3 defeat at the Bernabeu saw the standings at the top of the Primera Division became all the more crowded, with just one point separating the top three, who consist of the 'Clasico' rivals and the surprise party in the race, Atletico Madrid.
Atletico lead the way on head-to-head from Real, with Barcelona a point adrift, and nine rounds of games remain.
Wednesday's games could see a further twist, with Barcelona and Atletico both at home to mid-table opposition in Celta Vigo and Granada, respectively, while Real face a testing trip to in-form Sevilla.
With five consecutive wins in the league, Sevilla are a team to take seriously. They stand fifth on the back of that streak, and Ancelotti will need to prime his side accordingly.
But the Barcelona game remains high on the agenda in Spain's capital, and it was left to the Italian coach to face more questions on Wednesday about his team's loss, which came on the back of the dismissal of Sergio Ramos midway through the second half.
"We shouldn't look for someone to blame for the defeat," Ancelotti said in his press conference, according to the Real website.
"It came after several unbeaten games and we have to look forward. The team was fine before the Clasico and it remains fine afterwards. We are fighting in every competition.
"In the league, we have the same points as Atletico and one more than Barcelona.
"We have to look at the games coming up, especially tomorrow's game. It's clear that we intend to forget the last game and prepare well for Sevilla, which is a very important game for this season. The team is training well and in good spirits, ready to win the next game."
Ancelotti labelled Sevilla "a dangerous team", which is unmistakable.
Their coach, Unai Emery, said: "If we are fifth, it's thanks to the efforts of all the team, and our only, only, only objective tomorrow is to stay fifth."
That is guaranteed, at least in the short term, given the teams that can pass them, Villarreal and Real Sociedad, do not play until Thursday.
But Emery's point was clear, and he is determined for his players not to slacken off having put themselves in a strong position to qualify for Europe. They are through to the quarter-finals of this season's Europa League and have a taste for it.
The former Valencia boss is cautious though, wary of Madrid's quality.
"The individuals for Madrid can make the difference," Emery said, according to his club's website. "With the defeat the other day, they'll maybe come with a little bit of doubt, which can disappear at any moment because they are very good.
"To beat Madrid we'll have to give a perfect performance in all senses, in attack and defence."