Spurs welcome the Saints to White Hart Lane on Saturday afternoon looking to register a much-needed victory that will keep the London club in the hunt for Champions League qualification.
Key to that aim, of course, will be the performance of Gareth Bale. The Welshman is one of many players - like Theo Walcott, Alex Oxlade-Chamberlain, and Adam Lallana - who have come through the Southampton youth ranks and gone on to make a big impact in the Barclays Premier League.
That has not gone unnoticed by Villas-Boas, who rates the south-coast club's youth system so highly that he thinks Southampton have done for English football what Barca have done for the Spanish game in terms of youth development.
Comparing Southampton to La Masia - where Lionel Messi, Xavi, Andres Iniesta and Cesc Fabregas honed their skills - may seem a little far-fetched for some, but Villas-Boas may have a point considering Bale is now regarded as one of the best players on the planet.
The Spurs boss said: "Sometimes there are clubs that keep developing and nurturing players, then there are clubs that go for scouting.
"In Portugal, Sporting Lisbon is the development club and great players have come out of that school.
"They haven't produced the results in the first team as they wanted in the modern era. Porto invest in a later stage and don't develop the player.
"Southampton falls into the same category as Sporting Lisbon, like Barcelona, which is to nurture.
"But it is a great school of development - a bit like West Ham."
While Southampton and West Ham have churned out a number of top-quality players in the last two decades, Spurs have struggled to keep up with the pace.
Up until this year, Ledley King was the only Tottenham player who came through the youth ranks and made an impact on the first team.
Steven Caulker, Andros Townsend and Tom Carroll all have bright futures ahead of them with Spurs, it seems, and the club's development team currently top the newly created Under-21 Elite Group league.
"Tottenham has a great school with great young players," Villas-Boas said.
"The biggest jump for a young player is from the development team into the first team, and that gap they can try to bridge with the Under-21 league.
"But it's the first year and it needs time to get established. In the end I think it will allow players to get into the first team a little more often."
Bale's development in to a world-beater has been underlined by a successful week in which he has picked up a trio of prestigious accolades.
On Thursday the 23-year-old added the Football Writers' Association (FWA) Footballer of the Year award to his trophy cabinet just four days after he won Player of the Year and Young Player of the Year at the Professional Footballers' Association (PFA) ceremony.
Despite landing his latest prize, the Welshman's mind is firmly focused on securing victory against Southampton in what will be his 200th game for Tottenham.
"It's obviously great to get the 200 games under your belt and to make it against Southampton makes it even more special," said Bale, who scored in the reverse fixture, which Spurs won 2-1 last October.
"Hopefully I can make it even more special with a win.
"Southampton mean a lot to me. I was there from nine years old until I was 17 so it's a club close to my heart and I still have a lot of friends down there.
"But no matter who the opposition is we need to win and we need to put ourselves right in contention.
"It will be good, obviously, for us to win, but if I do score I will be muting my celebrations again like last time."