A section of disgruntled Villa supporters are planning to demonstrate against manager Alex McLeish prior to their Barclays Premier League clash with title-chasing City at Villa Park.
But whatever their dissatisfaction, Mancini believes the mood will have changed by kick-off and does not think the Villa players will be unsettled.
When asked if City could capitalise on any uncertainty, Mancini said: "We would hope to but I don't know this.
"It won't change our opinion, it will still be a difficult game. They lost their last game against Newcastle and so we will need to play a good game if we want to win against Aston Villa.
"When the game starts their supporters will support their team, always."
Mancini was heavily critical of himself after City were beaten at Everton in their last away game.
The Italian took the blame for the loss by saying he underestimated the Merseysiders and did not prepare the team properly.
He insists he and the players cannot afford to repeat any such errors as they attempt to hold off the challenge of Manchester United over the closing months of the season.
He said: "I know what my job is but I am human. Sometimes I can make mistakes, like the referee, like the players.
"Everyone can make a mistake sometimes. It is important the players understand this and they put everything on the pitch.
"When you think that you have made some mistakes, after you prepare better.
"If you made some mistakes, I don't think you can do the same mistakes against Aston Villa."
The 1-0 loss at Goodison Park continues to rankle with Mancini as, despite his admission of errors, he still feels City's performance warranted more.
He said: "We didn't deserve to lose that game but there are some moments you cannot win and, for this reason, sometimes we should understand if we can't win, we should not lose."
City have been title favourites since their 6-1 thrashing of United at Old Trafford last October but have been unable to pull clear at the top.
In the past two months they have lost at Chelsea, Sunderland and Everton as well as dropping points at West Brom.
They got their campaign back on track with a comfortable 3-0 defeat of Fulham last weekend but the battle remains tight.
He said: "For us it was really important to beat Fulham with a clean sheet.
"But I think every game from now until the end will be very difficult.
"Sometimes you play against top clubs, sometimes you play against teams playing for relegation, so for this reason I think it will be very hard.
"We know the season is long and we have another 14 games, every game is difficult."
Mancini also intends to ignore the rivals to concentrate solely on City's own performances.
Mancini, who claimed he "didn't see" United's 3-3 draw at Chelsea last week, said: "We can't think about the other teams. We can lose our strength by thinking about the other teams."
City are boosted by the return of captain Vincent Kompany from a knee injury while Samir Nasri is also fit after a groin problem.
Striker Mario Balotelli is again missing as he completes a four-match ban for a stamp on Tottenham's Scott Parker.
Mancini expects to have midfield inspiration Yaya Toure and his brother Kolo, the defender, back from the African Nations Cup for next Thursday's Europa League trip to Porto.