City imposed the fine after finding Tevez guilty of gross misconduct on December 22.
Tevez subsequently appealed against the punishment but that was thrown on January 7 after a hearing involving two City directors, Marty Edelman and Mohamed al-Mazrouei.
However, as with the disciplinary action brought against him after his non-appearance in Munich last September, Tevez had the option of taking the matter to the Premier League and Press Association Sport understands he has done just that.
Back in September, the 27-year-old succeeded in having a fine reduced from six weeks' wages to two, a saving of approximately £800,000.
In this instance, City feel their case is watertight given the punishment related to Tevez's presence in Argentina, where he has remained since November 7.
With it appearing increasingly likely Tevez will still be a City player after the end of Tuesday's transfer deadline, his assimilation back into Roberto Mancini's squad does not look like being straightforward.
The Premier League have confirmed they will deal with Carlos Tevez's appeal against a six-week fine imposed by Manchester City "at the earliest available opportunity".
"The Premier League received correspondence from Carlos Tevez last night giving notice of Mr Tevez's intention to appeal against a disciplinary sanction imposed on him by his club, Manchester City," the Premier League confirmed this afternoon.
"Premier League rules allow for players to appeal disciplinary decisions under Section K and the Premier League Board will consider this request at the earliest available opportunity."