With players like Luis Suarez, Andy Carroll, Charlie Adam, Stewart Downing, Jose Enrique, Jordan Henderson and Sebastian Coates arriving at Anfield this summer, they could get their first taste of the derby.
But Dalglish is confident the derby experiences the players have had in their previous clubs will stand them in good stead.
"I think Rangers against Celtic will have given Charlie a good grounding. For Andy, the north-east derby isn't to be undermined either, and for Stewart Villa against Birmingham isn't the smallest of derbies, is it?" he said, according to the club's official website.
"They may not have experienced one of these derbies before, but everybody has experienced a derby of some sort.
"Even for Luis (Suarez), the derby he's been involved in in Holland will have been frenetic as well. Every derby starts the same way.
"I don't think there is anybody who will be involved with us on Saturday who would undermine or be surprised by what they come up against.
"That's very important and although they might not have experienced the derbies here, they have somewhere else and they were equally as important to them when they were playing in them as what this one is."
The Reds boss, who is no stranger to Merseyside derbies during his playing days, knows how important the game is not just to the club - but for the people as well.
He added: "We know what a victory in a derby means to the people of Liverpool and we know how much elation the players would get from it.
"There are some players who haven't played in it before that might play on Saturday, but I'm sure they understand what this football club stands for."