The system will be tested at the Hampshire FA Senior Cup Final between Eastleigh FC and AFC Totton on May 16. A second fixture will be confirmed in the near future.
A second system developed by German/Danish firm GoalRef's system will also be tested in two separate matches - possible a Danish Superliga fixture and a forthcoming international friendly.
If they pass all the tests, the systems will be given the green light by the International FA Board on July 2.
FIFA said the tests in live matches would be monitored only by observers from EMPA (Swiss Federal Laboratories for Materials Science and Technology) and not the referee.
A statement said: "Critically, only the EMPA observer in the stadium will have access to the GLT system readings, and the system will NOT be utilised by the match officials.
"Therefore the GLT system will have no influence on the outcome of the matches in which the system is being tested."