Speculation has been rife over the west Londoners' managerial set-up since Friday's shock announcement of Felix Magath as new first-team manager.
The announcement brought an end to Meulensteen's 75-day spell at the Craven Cottage helm, but, despite him claiming he had been "released", he remained contracted as head coach.
However, the Dutchman has now formally left Fulham alongside four other members of the coaching staff.
Former Charlton and West Ham manager Curbishley has left his role as first-team technical director, while Wilkins has vacated the post of assistant manager.
Mick Priest and Jonathan Hill have also left the Whites, while Magath has brought Tomas Oralin as first-team coach and named Werner Leuthard conditioning coach.
"I am very grateful to Rene, Alan and Ray, as well as Mick and Jonathan, for their commitment to Fulham," Fulham chairman Shahid Khan said in a statement.
"Their efforts were admirable and appreciated, and I wish them the best.
"Felix has come to Fulham with purpose and exceptional confidence, and I know he is extremely pleased to have Tomas Oral and Werner Leuthard join him for the mission ahead.
"From today forward, the focus is singularly on winning football matches and remaining in the Barclays Premier League. The work has begun."
Magath's first game comes away at West Brom this Saturday - one of 12 matches left remaining for the bottom-placed Cottagers, who lie four points adrift of safety.