The Turin giants suffered a 1-0 reverse at Roma in the quarter-finals of the Coppa in midweek.
The setback was only Juve's second defeat in Italy this season and their first loss in 2014.
Antonio Conte's men return to the Stadio Olimpico for the second time this week determined to make amends.
"We face a difficult game against Lazio," Peluso said. "They are going through a good run of form while we go into the encounter on the back of a defeat.
"In the Coppa we only lost because of certain episodes but the time has come to bounce back and not making any more mistakes."
Juve beat Sampdoria 4-2 last weekend to clinch their 12th consecutive league triumph and go eight points clear of Roma at the top of the standings.
"We are growing as a team and this is the merit of our coach," Peluso said.
"We still have the scudetto and the Europa League to look forward to and we have a good chance to win both."
Lazio won the Coppa last season and take on Napoli next week in the quarter-final stages of the competition.
In the meantime, the Biancoceleste, who are unbeaten in 2014, will be looking to avenge their 4-1 league debacle to Juve back in August.
Lazio are ninth in the standings after last weekend's 3-2 triumph at Udinese, their first win on the road this season.
"My team showed great determination at Udinese," Lazio coach Edy Reja said. "We will need to show the same hunger and will if we are to surprise Juventus."
Lazio will not be at full strength with midfielder Ederson sidelined for the remainder of the season with a right thigh injury.
Moreover, defender Stefan Radu is still out injury while midfielder paid Ogenyi Onazi and Senad Lulic are both suspended.
Juventus are without defender Giorgio Chiellini, who must serve a one-match ban.