Barca this week announced plans to either redevelop their current Nou Camp home or move to land owned by the university adjacent to the Avinguda Diagonal in L'Hospitalet.
The club are hoping to increase capacity from the current 99,354 to 105,000.
However, the University on Thursday released a statement in which it denied holding any talks with the Spanish champions and reiterated its unwillingness to sell any land.
The statement on www.ub.edu read: "With regard to the stories that have been reported in recent weeks about the new movement of the FC Barcelona stadium, and what took place at the board meeting on Monday, the Universitat de Barcelona asks not to be included in this debate.
"The position of UB remains the same as expressed in its last communication of March 15, 2012.
"In the case that FC Barcelona expressed its interest in any part of the university, the rectorate would tell them the position of UB on this question."
In 2012, when it was first reported Barca had earmarked the land as a possible site for a new ground, the University stated: "UB does not do property negotiations and is not disposed to sell any of its assets."
On Monday, Barca board spokesman Toni Freixa claimed the club would make a decision on whether to stay at the Nou Camp or build a new stadium early in 2014.
He told their official website Barca had two choices: "The construction of a new stadium on the land on Diagonal, property which belongs to the University of Barcelona, or a profound remodelling project that would constitute a new stadium keeping the current structure of the Camp Nou.
"Both would have a capacity of 105,000 spectators, the stadium would be covered, there will be a construction of a new Palau with a capacity of 12,000 spectators, an adjacent court with a capacity of 2,000 spectators, 6,000 new parking spots and access to the Ciutat Esportiva of the Miniestadi.
"We've made advances, we have all the information and we're in a position to make a decision."
Despite the sizeable financial outlay that would be involved, Freixa insisted any project would not harm the club, adding: "It needs to be viable from a technical perspective, urbanist and economic. We would never submit a project that would endanger the sustainability of the club."