The Westphalians have fallen three point behind their local rivals recently, but victory in front of over 80,000 fans at the Westfalenstadion would take them back above Sami Hyypia's men in pursuit of league leaders Bayern Munich.
"We're very ambitious and we're not giving anything away," Klopp said. "We've got four big games to come before the winter break, and we'd like to win all of them."
Leverkusen will also be keen on the points to reinforce their current status of Germany's second strongest side.
They will also be out to prove that they can match one of Europe's best, according to Klopp, who expects them to be particularly motivated after losing 5-0 to Manchester United 10 days ago.
"I know they feel they belong in this category and they will want to show this quality," he said.
"They will be out for a bit of revenge, even though they don't have anything to put right against us in particular.
"We're going to have to be ready for that."
Klopp could be without Germany international Marco Reus, who he may be forced to risk even if he is not 100 per cent fit.
Due to a shortage of players, and the importance of the game, Klopp may have to drop his usual position when it comes to a player's fitness in Reus' case.
"Usually they need to train twice if they are to be even considered, but sometimes it's enough if they just turn up for breakfast," Klopp said.
Simply turning up is not going to be anywhere near enough for Leverkusen to take the points away with them, on the other hand.
However, Hyypia believes they will rise to the occasion and perform as they did in Munich earlier this season, when they came away with a draw.
"Everybody loves being involved in games like these," said the Finn. "The atmosphere is always fantastic there and it's going to be our task to quieten them down.
"We've got to believe in ourselves. I believe that we can win in Dortmund. If we don't believe in it, then we'd be better off staying at home and watching a film on TV."
Leverkusen will be without Germany international Sidney Sam, who is a doubt for the rest of the first half of the season with torn muscle fibres.
However, Stefan Reinartz and Sebastian Boenisch are both set to return.