Germany's neighbours need to avoid defeat to keep their qualification hopes alive in a group which sees the Republic of Ireland and Sweden all vying for second place behind Germany.
All three of Germany's closest challengers are five points adrift and an Austria victory would set up a tense finale in Group C.
With so much at stake Bierhoff is expecting a real battle at the Allianz Arena.
"Games against Austria are always emotional occasions," he said at a press conference.
"The Austrians are incredibly motivated to throw a spanner in our works.
"We have a great deal of respect for them. They have developed under a good coach and have 10 players in their squad who play in the Bundesliga.
"We are warned, but we are in front of our own fans and want to show them that Austria have no realistic chance against us."
Germany are without the injured Lukas Podolski - who will also miss their final qualifiers next month - Bastian Schweinsteiger, Mario Gotze and Ilkay Gundogan for Friday's clash with Austria and the trip to the Faroe Islands four days later.
"Of course that's frustrating," said Bierhoff. "Fortunately, we have enough good alternatives, so I'm not too concerned for the Austria game."
Germany's defence has provided enough cause for concern recently, conceding four against Sweden and the United States and three against Paraguay.
"If you look at the statistics, then we have conceded a lot of goals, but that is not purely a problem with our defence but with our whole team," said midfielder Sami Khedira, who admits he must provide more support to the beleaguered back line.
"All 11 players have got to be more compact and that way the defence does not come under so much pressure.
"We want to try that against Austria and if possible keep a clean sheet."
Khedira will form a partnership with Mesut Ozil in the Germany midfield, although that combination no longer exists at Real Madrid.
Ozil joined Arsenal on transfer deadline day, leaving Khedira as the only German in the Merengues' squad.
"Personally, this transfer is very regrettable," said Khedira. "We are good friends and have even strengthened our friendship in Madrid.
"His departure is also a loss to us in a sporting sense because Mesut is a player who can decide games by himself.
"However, we've got to accept his decision."