Villas-Boas said he hoped and expected Ferdinand not to snub Terry, despite allegations the Rangers defender was the victim of a racist slur from the England captain during October's Barclays Premier League game between the sides.
The players square off on Saturday for the first time since then, and just four days before the court case begins in which Terry stands accused of abusing Ferdinand.
Ferdinand is reportedly agonising over whether to shake Terry's hand before the match, amid fears it could be viewed as hypocritical.
Asked if it was important he did so, Villas-Boas said: "Extremely important, extremely important."
He added: "This game is based on good values more than anything else.
"These players should continue to promote these good values."
Villas-Boas confirmed the matter had been discussed with Terry, who denies having racially abused Ferdinand.
The Chelsea boss said: "Everybody has been having conversations, from the top, to the players, to the manager."
Mark Hughes refused to add to the pressure on Anton Ferdinand to shake John Terry's hand before QPR's FA Cup clash with Chelsea after Andre Villas-Boas claimed it was "extremely important" he did so.
Rangers boss Hughes said it was entirely Ferdinand's decision whether to snub Terry during the pre-match handshake prior to Saturday's west London derby at Loftus Road.
Hughes said on Friday afternoon: "If he feels it is not appropriate, that has to be his own decision.
"It won't be influenced by myself or the club."
Neither Villas-Boas nor Hughes had any fears over their respective defenders' mentality going into Saturday's game.
Villas-Boas said of Terry: "I have to count on the player, and the player is fit and available for the game."
Hughes said: "Anton is very clear in his mind, as I am, that irrespective of what surrounds the game, and the speculation and conversations, that he is fit, he is well and able to play."
Hughes played down reports Ferdinand had spent £30,000 on personal security to ensure a trouble-free journey to Loftus Road.
He joked: "We're actually in a hotel tonight and travelling to the ground by coach so it is 30 grand he didn't have to spend."
Chelsea have already vowed to ban any fan found racially abusing Ferdinand from Stamford Bridge, including those who sing the now-notorious "Anton Ferdinand, you know what you are" chant.
Hughes suggested QPR stood ready to eject anyone from Loftus Road heard doing so on Saturday.
He said of the chant: "I think we understand what is meant by that."
He added: "If there is inappropriate chanting or behaviour then you get ejected from the ground and rightly so."
Extra police have been laid on for a game which will kick-off at 12pm in a bid to limit any trouble, while both clubs issued strongly-worded statements this week warning supporters to behave.
Villas-Boas admitted there were also "extra responsibilities" for both sets of players to keep calm amid what could be a hostile atmosphere on Saturday.
Chelsea had two players sent off in October's league meeting but Villas-Boas insisted there was no need to warn his squad about their behaviour prior to kick-off tomorrow.
"I won't speak to the players specifically about that before the game, not at all," said the Blues boss, who predicted the match would pass off without trouble.
"The environment was outstanding the last time we played there at Loftus Road, so I expect it to be the same."
Hughes said: "We as a club obviously have spoken quite closely with Chelsea and the police.
"I just hope everybody is sensible and enjoys the game for what it is, a fantastic cup tie that we're all looking forward to.
"We basically want to be talking about the football and what happens on the pitch."