The Serie A leaders are considered overwhelming favourites in their last-32 tie.
However, the Italians must be wary for their last meeting against Turkish opposition ended in tears when a 1-0 defeat at Galatasaray knocked Juve out of the Champions League back in December.
Trabzonspor, meanwhile, cruised to the knockout round of the Europa league, topping Group J with an unbeaten record.
"We cannot forget that Trabzonspor finished ahead of Lazio in the group stages of the competition," Conte said in Wednesday's press conference.
"They have very good players and they have experience. We will have to be focused from start to finish."
Juve go into the encounter on the back of Sunday's 3-1 triumph over Chievo that lifted them nine points clear of Roma, who have a game in hand.
Conte has revealed he will make changes to his starting XI on Thursday in view of their derby against Torino on Sunday.
"We want to honour the Europa League as best as we can," Conte said. "But looking at our schedule, I realize that in 53 days my players will be involved in 16 games.
"Hence, I have to make changes and make sure that all my players are ready for this challenging schedule."
Juve will be without injured defender Giorgio Chiellini while Spanish striker Fernando Llorente is expected to rest.
Daniel Osvaldo is set to earn his first start since joining Juve on loan from Southampton in January and will play alongside striker Carlos Tevez, who is looking to score his first goal in Europe since April 2009.
Trabzonspor travel to the Juventus stadium, the venue of this year's final, looking to put their domestic woes behind them.
Their poor run of results led to Trabzonspor coach Mustafa Akcay being dismissed earlier this month with club legend Hami Mandirali replacing him.
Trabzonspor are ninth in the Turkish Super League and have clinched just one win in their last eight league games.