Mexico have already qualified for the six-team final section and both the United States and Guatemala would join them if they drew in Kansas City. Jamaica must beat Antigua & Barbuda and hope for a win either way in the States to have any chance of progressing.
The USA need to improve their form after the underwhelming 2-1 win away to Antigua & Barbuda on Friday night, while Guatemala overcame Jamaica 2-1 and want to be in the final stage.
"A lot of us have really enjoyed seeing the evolution of this group and to play a qualifier like this in our country, we know it is an American crowd, a big crowd, and it's going to be a final," midfielder Michael Bradley told USsoccer.com.
In Group B, Costa Rica look best placed to finish runners-up to Mexico, with a win over already-eliminated Guyana certain to secure their place in the final round. El Salvador need an unlikely win away over Mexico in Torreon to have any hope of going through at Costa Rica's expense.
"We have to try. There is little chance but we'll try to clinch the goal," El Salvador coach Juan de Dios Castillo told reporters in San Salvador, before the flight to Torreon.
Forward Jonathan Aguila added: "We know the match will be tough, but we should not lose faith. We'll do our best to win. To God, nothing is impossible."
Costa Rica forward Alvaro Saborio, meanwhile, called his team-mates to commit themselves ahead of the match against Guyana: "All the guys got the message. We hope we can win in order to continue our road to the World Cup."
In Group C, everything will be defined between leaders Panama, Canada and Honduras.
Panama and Canada share top spot on 10 points, while Honduras are currently third on eight.
The Panamanian side will visit Cuba in Havana, while Canada and Honduras face a head-to-head fight for qualification.
Cuba will be without four players who deserted before the previous qualifier against Canada. They are midfielder Raisender Fernandez-Cervantes, forwards Elier Cordovez-Gonzalez and Maikel Chang-Ramirez, and keeper Odisdel Cuper-Despaine.
"We will go to Cuba to win the match," Panama coach Julio Dely Valdes told reporters.
"We are focused on this match because we want to finish this round on top of the group and ratify what we have been doing so far."
"We don't know what we are facing because Cuba know they are playing for nothing and maybe want to think of the future with young men."
Finally, Honduras boss Luis Fernando Suarez expects a tough game against Canada.
"We already know what we have to do. We know Canada play very well defensively.
"We believe in ourselves. We don't have to change anything. We'll study how to hurt them at home."