Both sides will finish in mid-table, but that is no reason for them to take this final Bundesliga challenge lightly.
"It's our last league game and our primary aim is to end it with a win in front of our own fans," said coach Bruno Labbadia, whose side's season does not end tomorrow [Saturday].
They still have a DFB-Pokal final against Bayern Munich to look forward to two week's time, which is why tomorrow's game has added importance.
However, Labbadia admits his side will have to raise their game when they travel to Berlin on June 1.
"It's hard to find a good sparring partner anywhere in Europe who has the same ability which Bayern have," he told reporters.
"Our priority is to remain in our rhythm."
Mainz will do their best to give Stuttgart as stern a test as possible with their coach Thomas Tuchel rejecting claims his side are already thinking about their summer break.
"I still recognise that we want to win," he said. "It's a shame that the impression is that we are winding things down.
"The players are giving it their very last. The feeling you get from winning a game is unrivalled."
Niki Zimling is back for Mainz, who are likely to be without Elkin Soto.
Nicolai Muller, who has earned his first Germany call-up this week, will rule the roost in the midfield while Bo Svensson is suspended and Jan Kirchhoff is injured.
Cacau is back in training for Stuttgart, but he will not make his first appearance of the season.
However, on an interview on his club's website, the Germany international revealed his target is to appear in the DFB-Pokal final.