The hosts are four points adrift of sixth-place Freiburg, with Mainz a point further back after recent defeats.
While the winners on Saturday may still have a mathematical chance of breaking into the top six over the final two games of the season, realistically it now is all about ending the season on a high.
And that alone should be motivation enough, according to Hannover boss Mirko Slomka and his Mainz counterpart Thomas Tuchel.
"I don't accept anything half-hearted," said Slomka. "Not in training and not in a game either.
"We have a duty to our club and to our fans."
Tuchel added: "Motivation has never been a problem for us. We are not going to start any experimentation."
Tuchel may have to shuffle his pack, though, with Elkin Soto, Andreas Ivanschitz, Heinz Muller and Niko Bungert all due to face late fitness tests.
However, he wants his side to build on the second-half performance in the goalless draw with Eintracht Frankfurt last weekend.
"It was an honourable performance and we can take heart from that," he said.
Slomka welcomes Christian Schulz back after he recovering from a back injury, but Manuel Schmiedebach is suspended and Johan Djourou still unavailable.
Slomka's aim is to gain revenge for a 2-1 defeat in the reverse fixture.
"We want to turn that result around," he said.