The hosts' five-year Bundesliga stay is at serious risk of coming to an end next month as, with four games to go, they are six points from safety and three behind Augsburg, who hold the relegation play-off berth.
"We're going to throw everything we've got into the mixer in order to survive," Gisdol said.
A 5-0 defeat at Bayer Leverkusen a week ago was a setback, but Gisdol has been encouraged by what he has witnessed since that game.
"We mustn't read too much into the Leverkusen result," he added. "The impressions I have got from training have been good."
Hoffenheim will be without Eugen Polanski on Saturday and for all but the final game of the season because of a three-match ban for his red card against Leverkusen, and the length of the suspension has angered Gisdol.
"To begin with, I thought it was a printing mistake," he said.
"It is a drastic punishment, particularly when an Augsburg player only got one game for the same kind of incident.
"But we've just got to accept the punishment and not moan. I expect other players to take on the responsibility."
Nuremberg coach Michael Wiesinger expects his side to bounce straight back from the disappointment of losing to local rivals Greuther Furth last weekend.
That result ended any lingering hopes the Bavarians had of qualifying for Europe next season, but Wiesinger said: "We need to show a reaction, but we shouldn't just be talking about this, we actually have to show it too.
"We had a chance in the derby to turn a good season into a very good one, but we gave it away easily.
"We can still make up for that in our remaining four games."
Wiesinger is without the suspended Per Nilsson while Timm Klose is out due to concussion.