Last weekend's victory reduced the arrears on Augsburg in the relegation play-off berth to a single point while only six points now separate them from Fortuna in 15th.
Gisdol is therefore calling on his side to sting the Wolves to give them a fighting chance of survival going into the last five games of the season.
"We've got to hunt our opponents like a swarm of bees," he said on his club's website.
"I want to see us build on the tentative signs we showed against Dusseldorf and be even more effective against Wolfsburg.
"We want to carry on along the path we started against Dusseldorf, step by step.
"The level of expectation must not get too big, though. You aren't going to hear any proclamations from us. We're just going to work, work, work."
Wolfsburg also still have work to do before securing their top-flight status for another season, but with a DFB-Pokal semi-final against Bayern Munich on the horizon, they could be forgiven for taking tomorrow's match a little lighter.
Their coach Dieter Hecking insists that will not be the case, though.
"Our focus is quite clearly on the Hoffenheim game," he said. "From 17:20 tomorrow [Saturday], we can then deal with the cup.
"Hoffenheim are going to be very demanding and they are a team with many strong individuals.
"Certainly nobody expected them to be where they are now, but they're going to give it everything in their remaining games to reach at least 16th place."
Wolsburg are without the suspended Fagner, but Vieirinha is available again.
Koen Casteels has returned to training for Hoffenheim and should take his place in goal with Heurelho Gomes ruled out for the rest of the season.