Dortmund won seven competitive games out of seven prior to Wednesday's 2-1 defeat in Napoli and can increase their advantage at the top of the Bundesliga to five points over Bayern Munich, who travel to Schalke in the evening.
"We want the points there to stay at the top of the table," director of sport Michael Zorc told the club's website.
"We've never had it easy in Nuremberg in recent years, but we have mostly been successful there.
"Our challenge will be to find our way back into the Bundesliga after an away game in the Champions League which didn't bring success.
"We've got to be 100% focused on this game."
Coach Jurgen Klopp has several doubts surrounding his starting XI, not least after seeing Mats Hummels limp off against Napoli and Neven Subotic pick up a cut just above his eye.
Sven Bender filled in as a makeshift central defender on Wednesday with Sokratis Papastathopoulos ruled out injured.
For all his concerns at the back, Klopp has a plethora of options up front, allowing himself the luxury of leaving Pierre-Emerick Aubameyang on the bench against the Serie A side, and the Gabon international could return on Saturday.
Nurnberg coach Michael Wiesinger saw Dortmund's Champions League game on the television and he now cannot wait to experience the kind of atmosphere they must have felt at the Stadio San Paolo when a similar size crowd fills the Frankenstadion.
"The thought of playing in front of 50,000 fans is pure motivation for us," he said at a press conference.
He will give Robert Mak a late fitness test, although there will be no room in the squad for Hanno Balitsch.