The Hong Kong outfit are currently third in the group, a point behind Tuesday's opponents, and will be buoyed by recording their first-ever win in the competition in their last game against Yangon United - a 2-1 victory.
However, Chu is under no illusions that his side will have their work cut out for them against Chonburi, a side he believes are "the best in the group".
"We are prepared and ready but we know Chonburi are the best team in our group," Chu said on the AFC's official website.
"However, we are going to play our best to gain the three points, although I would be happy with at least one point."
Citizen's cause will be helped by the injury problems currently plaguing Chonburi, something coach Withaya Laohakul admits is worrying him.
"We are a bit concerned that we have some key injuries with our starters, especially on both flanks," Withaya said.
"However, we are still confident with what we will have on the field and also the work the players have been putting in towards finishing our chances."
Group G's second-placed team Home are also facing selection problems of their own ahead of their game against Burmese side Yangon, with Protectors coach Lee Lim-saeng revealing he only has 15 players to choose from on Tuesday.
"We have had problems with injuries and we were only able to bring 15 players with us as a result of that," Lee lamented.
In Group C, Al Kuwait are set to welcome back Ali Al Kandari and Nasser Al Qahtani for their clash against Al Ahed.
Both players missed the last match against VB, which ended in a 2-2 draw, but despite welcoming two of his key players back, Al Kuwait coach Mohamed Abdullah has called for caution ahead of Tuesday's game.
"The match against Al Ahed is very important and at at the same time it will be very hard for our team because we are playing away and in front of the Lebanese league champions, who performed well in their previous two matches," he said.
Elsewhere in the group, Maldives' VB take on Saudi Arabia's Al Ettifaq.
Al Shorta will be looking to continue their perfect start to the campaign in Group E when they entertain pointless Al Tilal, while Al Zawra'a and Safa do battle for second place.
Finally in Group A, Al Qadsia host Al Faisaly while Al Ittihad travel to play Al Suwaiq.
Tuesday's AFC Cup Fixtures
Al Qadsia vs Al Faisaly
Al Suwaiq vs Al Ittihad
Al Ahed vs Al Kuwait
VB vs Al Ahed
Al Shorta vs Al Tilal
Al Zawra'a vs Safa
Chonburi vs Citizen
Yangon United vs Home United