Saudi Arabia need a victory to ensure they progress to the next round alongside already-qualified Australia from Group D, with either Thailand or Oman in position to take advantage should they slip up.
Australia won 3-1 in Dammam last September and Osieck revealed his side would be going for another win, despite having top spot already sewn up.
"We take it very seriously, it's an international game and it's a great challenge for us to again play on the international stage," Osieck said.
"So we are focused on giving a very good game, having a great performance and I know from all our people we're going to expect a good turnout so that is another obligation for us to really perform well and I think I'm really looking forward to it."
Captain Lucas Neill added: "There's no such thing as a practice match and the manager has already mentioned every time we play we're always playing for our places so this game is no different.
"Why would we want to come and play a game, an international, and not want to win it?
"We're playing for a bigger prize now, which is going to Brazil (for the World Cup finals), and we have to prepare now.
"This is a game that we want to win and we know they have to win, so if we find ourselves putting them under pressure we know that they will feel that and their country will feel that and we have to take advantage."
Oman host Thailand in the other game in the group, with Oman just one point behind Saudi Arabia in third and Thailand only a point further back.
South Korea need at least a point from their Group B clash with Kuwait in Seoul to guarantee progression, but only a win will likely earn them top spot.
South Korea head the group from Lebanon on goal difference, with Kuwait two points behind in third.
With Lebanon hot favourites to win away to the pointless United Arab Emirates, South Korea still have work to do.
"It's a one-off match where we must get a good result," captain Kwak Tae-hwi said on www.fifa.com. "We cannot afford to lose this one, and we should concentrate on defending so they cannot get what they want. We will and we must win."
Jordan and Iraq have already sealed the qualification spots from Group A.
First place is still up for grabs with both sides level on points and Jordan travel to China on Wednesday with Iraq hosting Singapore.
Group C is also all but done and dusted.
Uzbekistan need a point away to fellow qualifiers Japan to guarantee first place, with Tajikistan hosting North Korea in the other game.
Qatar need a point against already-qualified Iran to guarantee their progression from Group E, while a win would earn them top spot.
Even a defeat should see Qatar through, though, with third-placed Bahrain needing to beat Indonesia, hope Qatar lose and make up a nine-goal deficit.