Kelantan, who narrowly pipped Terengganu to the title last season, will be the first team to encounter the Singapore representative side, who are playing in the Super League under an agreement between the Malaysian and Singaporean Football Associations, on January 10.
Terengganu begin their campaign with a trip to Perak, while perennial challengers Selangor, who finished third last season, travel to reigning Malaysia Cup champions Negeri Sembilan on the opening day in what is likely to be a heated encounter.
Newly-promoted sides PKNS FC and Sarawak kick off against Johor FC and Kuala Lumpur respectively, while Terengganu-based T-Team entertain Kedah.
Fans eagerly anticipating the rematch between last season's top two will have to wait until February 11 when Kelantan host Terengganu at the Sultan Muhammad IV Stadium, while the first West Coast derby of the season between Selangor and Perak is due to take place on January 14.
Singapore fans looking to relive the Malaysia Cup days of the early 1990s will have to wait till February 7 to resume their old rivalry with Selangor at the Bukit Jalil Stadium, while the return leg at the Jalan Besar Stadium is scheduled for June 23.
Meanwhile, Sabah, buoyed by the signings of Australian duo Brendan Gan and Michael Baird, will be looking to improve on last season's 10th-placed finish. They start their season against Kuala Lumpur-based Felda United.
2012 Malaysia Super League Round 1 Fixtures
Negeri Sembilan vs Selangor
Perak vs Terengganu
Felda United vs Sabah
Sarawak vs Kuala Lumpur
T-Team vs Kedah
LIONSXII vs Kelantan
PKNS vs Johor FC