Andre Senghor and Fawzi Basheer scored for Bani Yas with Ghana international Asamoah Gyan, on a season-long loan from Sunderland, netting both Al-Ain goals, the second a penalty.
Al-Ain are now five points behind the table-toppers.
Ammar Salem scored a hat-trick and Rodrigo Careca and Mahmoud Hassan also netted as Al-Nasr won 5-4 away to Sharjah.
For the home side Marcelinho and Edinho both scored twice.
Grafite and Coelho Jaja scored for Al-Ahly Dubai in the 2-2 draw at home to Al Wahda whose goals came from Amer Omar and Eissa Abdulla Al-Saadi.