Saturday's draw in Brazil saw Japan drawn alongside North Korea, Uzbekistan and Syria in Group C of Asian qualifying, and while Zaccheroni's men - as reigning Asian champions - are still favourites to qualify, they face a tougher route than some of their counterparts.
Nonetheless, Zaccheroni believes that Japan would eventually have had to face established teams in the final stage of qualifying, and is looking to eliminate them sooner rather than later.
"Our group may seem a difficult group but they are opponents whom we must play sooner or later on our way to the World Cup finals," Zaccheroni said, according to the Asian Football Confederation's official website.
"So we will just go for it."
Zaccheroni did however concede that Japan's status as Asian champions would mean their opponents would be suitably prepared for the challenge they will pose.
"We are the Asian champions, so our opponents will be doing their homework and come at us firing on all cylinders, but we will just focus on each and every match."
Japan kick off their qualifying campaign at home to North Korea on September 2, before travelling to Uzbekistan just four days later.
Japan Football Association technical director Hiromi Hara believes these two games will go a long way in deciding the final group standings.
"Our first two games will be important.
National team goalkeeper Eiji Kawashima also conceded Japan have their work cut out for them, telling Kyodo News: "There are no easy groups. Whoever you play in Asia it is always a tough battle.
"The most important thing is that we all prepare well."
Japan put in an impressive showing at the World Cup last year, qualifying for the knockout stages after finishing second in Group E, before they were eliminated by Paraguay on penalties.