Hajime Moriyasu's side have suffered back-to-back defeats by Uzbekistan's Bunyodkor and China's Beijing Guoan at the start of the campaign and find themselves two points adrift at the foot of the table heading into the midway point of the group stage next week.
Bunyodkor eased to a comfortable 2-0 win at Hiroshima Big Arch on Matchday One before Piao Cheng scored with 11 minutes remaining in Beijing after Naoki Ishihara had pulled Sanfrecce level with a quarter of an hour remaining.
Sanfrecce's situation could have been even worse had Pohang hung on to all three points against Bunyodkor just over two weeks ago before being forced to settle for a second consecutive draw as Kamoliddin Murzoev netted with the last kick of the game in Tashkent.
The draw leaves Beijing and 2012 semi-finalists Bunyodkor two points clear at the top of the table ahead of Tuesday's meeting between the unbeaten pair in Tashkent.
Sanfrecce, though, did record a timely 4-0 win over Shimizu S-Pulse on Saturday thanks to two goals from 2012 J.League MVP Hisato Sato, while Pohang maintained their position at the top of the K-League with a 2-1 win over Chunnam Dragons.
In Group E on Tuesday, K-League champions FC Seoul and Japan's Vegalta Sendai also put their unbeaten records on the line, while China's Jiangsu Sainty entertain Thailand's Buriram United.
FC Seoul made an impressive start to their AFC Champions League campaign after easing to a 5-1 home win over Jiangsu at the end of February before sharing a goalless draw with Buriram last time out.
Sendai and Buriram sit just two points off the pace with Jiangsu recovering from their opening day defeat by FC Seoul by holding the J.League side to a goalless draw in China earlier this month.
Also on Tuesday, Al Ahli travel to Iran to face Hazfi Cup finalists Sepahan with the 2012 runners-up out to maintain one of only three remaining 100% records in the AFC Champions League this season.
Al Ahli followed up beginning their campaign with a 2-0 home win over Qatar's Al Gharafa as Oman striker Amad Al Hosni scored twice earlier this month as new coach Aleksander Ilic enjoyed a successful first outing in the AFC Champions League with a 2-1 comeback win over 10-man Al Nasr in Dubai.
Ilic had replaced Karel Jarolim, who had guided Al Ahli to last year's final, following the opening day home win over Al Gharafa.
Former finalists Sepahan, who secured a place in the Hazfi Cup final with after edging out Esteghlal on penalties on Saturday, are out to bounce back after losing 3-1 to Al Gharafa, who picked up their first win of the campaign to join the Iran champions in second place three points behind Al Ahli.
Also in Group C on Tuesday, Al Gharafa travel to Dubai to face play-off winners Al Nasr with the home side seeking their first points of the campaign after losing 3-0 to Sepahan on Matchday One.
Al Nasr, though, will be without captain Leonardo Lima after the Brazilian midfielder was sent-off in the first half against Al Ahli.
Finally, Saudi champions Al Shabab entertain fellow Group A leaders Tractorsazi Tabriz, while Al Jazira travel to Qatar to face El Jaish.
Both Al Shabab and Iran's Tractorsazi have posted unbeaten records after following up opening day home wins with draws against Al Jazira and El Jaish earlier this month.
El Jaish will be without suspended duo Mohamed Methnani and Ko Seul-ki, while Matias Delgado will miss Al Jazira's trip to Qatar after the Argentinean midfielder was sent-off in the closing stages of the 1-1 draw with Al Shabab.
Tuesday's AFC Champions League Fixtures
El Jaish vs Al Jazira
Al Shabab vs Tractorsazi Tabriz
Al Nasr vs Al Gharafa
Sepahan vs Al Ahli
FC Seoul vs Vegalta Sendai
Jiangsu Sainty vs Buriram United
Sanfrecce Hiroshima vs Pohang Steelers
Bunyodkor vs Beijing Guoan
Image courtesy of WSG